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KMID : 1005220130280030251
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Journal of Embryo Transfer
2013 Volume.28 No. 3 p.251 ~ p.256
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Effect of Hh-Ag1.5 Treatment on the In Vitro Development and Apoptosis of In Vitro Fertilized Embryos in Pigs
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Kwon Dae-Jin
Yeo Jae-Hun
Noh Won-Gun
Kwak Tae-Uk
Oh Keon-Bong
Ock Sun-A
Im Seok-Ki
Park Jin-Ki
Hwnag Seong-Soo
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Abstract
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The present study was performed to investigate the effect of Hh-Ag1.5, a small-molecule chemical agonist of SMOothened receptor, on the in vitro maturation and development of in vitro fertilized (IVF) embryos in pigs. Oocytes or fertilized embryos were cultured in a maturation or embryo culture medium supplemented with 0 (control), 25, 50 or 100 nM of Hh-Ag1.5, respectively. Although the maturation rate were not different among treatment groups, the blastocyst formation rate in the group treated with 25 nM Hh-Ag1.5 was significantly increased compared to other groups (P<0.05). While the highest dose of Hh-Ag1.5 (100 nM) did negatively affect to the embryo development and cell number in blastocysts compared to other groups (P<0.05), the apoptotic cell index in blastocysts was significantly lower in 25 and 50 nM groups than in control and 100 nM groups (P<0.05). The mRNA expression of the proapoptotic gene Bax and the ratio of Bax/Bcl-XL decreased in among treatment groups compared to control (P<0.05). The embryo quality related genes, Tert and Zfp42, were significantly decreased in 50 and 100 nM groups compared with control and 25 nM groups (P<0.05). In conclusion, the addition of 25 nM Hh-Ag1.5 to in vitro maturation and culture medium can enhance the developmental potential as well as quality of IVF embryos in pig.
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KEYWORD
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SMOothened , Hh-Ag1.5 , apoptosis , embryo quality , pig
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